Fig. 8: The impact of dsRNA formulation on spray-mediated control of Magnaporthe oryzae on Brachypodium distachyon leaves. | Communications Biology

Fig. 8: The impact of dsRNA formulation on spray-mediated control of Magnaporthe oryzae on Brachypodium distachyon leaves.

From: Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Fig. 8

Relative size of necrotic area on Bd leaves sprayed with Mo conidia and with naked Pmk1-dsRNA (1 ng/µL), chitosan-based Pmk1-dsRNA nanoparticles (NPs) or empty NPs. Control plants were sprayed only with conidia solution (CTR). Relative size of the necrotic area to the whole leaf was calculated at 5 dpi with ImageJ. (A) Simultaneous treatment with Mo conidia and naked or formulated dsRNA or empty NPs. B Sequential treatment with a time gap of one day between dsRNA/NP treatment and subsequent Mo inoculation. C Sequential treatment with a time gap of 7 days between dsRNA/NP treatment and subsequent Mo inoculation. The results of three independent repetitions were combined and box plots represent average with standard deviation. Statistical significance was assessed with Kruskal-Wallis test (p ≤ 0.05) and asterisks denote difference to the control group according to Dunn’s multiple comparisons test. Pairwise comparison in (C) was performed with Mann-Whitney test. (A, ****: p ≤ 0.0001; B, ***: p = 0.0003; ****: p ≤ 0.0001; C, **: p = 0.0083; ****: p ≤ 0.0001).

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