Fig. 2: NLRP7 knockout causes DNA damage and apoptosis in n-hESCs. | Communications Biology

Fig. 2: NLRP7 knockout causes DNA damage and apoptosis in n-hESCs.

From: NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing

Fig. 2

A Schematic of the chemical resetting of p-hESCs to n-hESCs. B Representative phase-contrast images of resetting n-hESCs cultures. Scale bars, 100 μm. C Immunostaining of general and naïve pluripotency proteins in p-hESCs and established resetting n-hESCs. Scale bars, 100 μm. D RT-qPCR analysis of general and naïve pluripotency markers in resetting n-hESCs. Data are shown as mean ± SEM. Student’s t-test. E Representative immunofluorescent images of n-WT and n-NLRP7−/− for the DNA damage marker γH2A.X (red). The nuclei were counterstained with DAPI and are shown in blue. Scale bar, 50 μm. BOX, Scale bar, 5 μm. F Quantification of the γH2A.X foci in the immunofluorescent staining in (E). Data are shown as mean ± SEM. Student’s t-test. G Representative immunofluorescent images of n-WT and n-NLRP7−/− for the Apoptosis marker active caspase 3 (red). The nuclei were counterstained with DAPI and are shown in blue. Scale bar, 20 μm. BOX, Scale bar, 5 μm. H Quantification of the Active Caspase 3 in the immunofluorescent staining in (G). Data are shown as mean ± SEM. Student’s t-test. I Representative phase-contrast images of n-WT and n-NLRP7−/− at P20. Scale bars, 100 μm.

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