Fig. 3: NLRP7 knockout affects human blastoids development.

A Schematic of human blastoids formation from n-hESCs. Pyramid shape, the microwell of aggrewell 400; Pink cells, primitive endoderm; Green cells, epiblast cells; Naïve embryonic stem cell medium (5i/L/A), Hypoblast differentiation medium (HDM), Trophoblast differentiation medium (TDM). B Representative phase-contrast images of cell aggregates at indicated time points during blastoid formation. Scale bar, 100 μm. C The diameter of blastoids derived from n-WT and n-NLRP7^−/−. Data are shown as mean ± SEM. Student’s t-test. D Representative immunofluorescence co-staining images of WT blastoids and NLRP7^−/− blastoids for trophectoderm marker GATA3 (red) and the epiblast marker OCT4 (green). Scale bars, 100 μm. E Quantification of the number of OCT4⁺ and GATA3⁺ cells in human blastoids (WT Blastoids, n = 14 biological replicates; NLRP7^−/− blastoids, n = 11 biological replicates). Box plots show median (center line), 25th and 75th percentiles (bottom and top of the box, respectively), and minimum and maximum values (bottom and top whisker, respectively). Data are shown as mean ± SEM. Student’s t-test.