Fig. 5: Both ESX-3 an ESX-4 are required for blocking phagosome maturation and phagosomal escape. | Communications Biology

Fig. 5: Both ESX-3 an ESX-4 are required for blocking phagosome maturation and phagosomal escape.

From: Deletion of ESX-3 and ESX-4 secretion systems in Mycobacterium abscessus results in highly impaired pathogenicity

Fig. 5

a Co-localization of Mab S, SΔeccC3, SΔeccC3::c, SΔeccC4, SΔeccC4::c, SΔeccC3/ΔeccC4, and heat-killed strains (red) with the acidotropic dye LysoTracker (green) in infected THP-1 cells at 20 hpi. Data are presented as means from three independent experiments (n = 9). Statistical significance was assessed using Tukey’s test: ***, P <  0.0001, ****, P < 0.00001. b Acidic compartments were visualized by confocal microscopy using LysoTracker, a fluorescent marker for acidic compartments, in infected macrophages. Co-localization of red fluorescent signals from Mab S, SΔeccC3, SΔeccC3::c, SΔeccC4, SΔeccC4::c, SΔeccC3/ΔeccC4, and heat-killed bacteria with LysoTracker was observed in infected THP-1 cells. Yellow coloring indicated co-localization of red and green labeling. c Percentage of infected macrophages containing at least one positively-stained phagosome (Gal-3+). The values represent the mean of 1000 infected cells analyzed from three different experiments (n = 10). P values were determined by ANOVA with Tukey’s test; ****, P < 0.0001. d IL-1β production during THP-1 infection by Mab S, SΔeccC3, SΔeccC3::c, SΔeccC4, SΔeccC4::c, SΔeccC3/ΔeccC4, and heat-killed strains. The data reflect mean values from three independent experiments, each in quadruplicate (n = 12). P values were determined by ANOVA with Tukey’s test; ****, P < 0.0001. Data are mean ± SD.

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