Fig. 3: Increased myeloid immune cells and interstitial macrophage populations in aged HPS mice.

a UMAP of myeloid lineage immune compartment following isolation and re-projection in 8- and 60-week-old HPS mice, focused on macrophages, dendritic cells, and monocytes. b Over representation analysis of biological processes that are upregulated in myeloid cells from 60-week-old HPS1-2 mice compared to age matched WT controls. c Over representation analysis of biological processes that are downregulated in myeloid cells from 60-week-old HPS1-2 compared to age matched WT controls. d Representative immunostaining of CD68⁺ cells in HPS mice, by age and genotype. Imaging experiments were performed on 50 µm thick PCLS from at least three independent biological replicates per age and genotype. Arrows indicate regions of increased CD68⁺ cell clustering. Scale bar = 100 µm. e Percentage of CD68⁺ cells per DAPI⁺ cells. Data are presented as mean ± SEM and were obtained from n = 3 8wk HPS2, 8wk HPS2-TG, 60wk WT, 60wk HPS1, 60wk HPS2, and 60wk HPS2-TG mice, and n = 4 8wk WT, 8wk HPS1-2, 8wk HPS1, and 60wk HPS1-2 mice. For each biological replicate, a minimum of three images were taken of lung parenchyma, and the percentage of CD68⁺ cells was calculated as the average of those images. P values were obtained by two-way ANOVA with Dunnett’s post hoc analysis. f Proportion of interstitial macrophages (MΦ) (CD45⁺CD11b⁺MHCII⁺CD64⁺CD24^-) in 8-week-old mice by flow cytometry, as a percentage of total CD45⁺ cells. Data are presented as mean ± SEM and were obtained from n = 3 each for HPS1 and HPS2 mice, n = 6 HPS1-2 mice, and n = 7 WT mice. g Proportion of interstitial macrophages (MΦ) in 60-week-old mice by flow cytometry, as a percentage of total CD45⁺ cells. Data are presented as mean ± SEM and were obtained from n = 3 each for WT, HPS1, and HPS2 mice, and n = 4 HPS1-2 mice. P values were obtained by one-way ANOVA with Dunnett’s post hoc analysis. N.S. = not significant.