Table 1 Primer and guide RNA sequences used for experimental validation on Neisseria gonorrhoeae and Streptococcus pyogenes gDNA
Species | gRNA name | PathoGD module | Forward primer | Reverse primer | gRNA spacer |
|---|---|---|---|---|---|
Neisseria gonorrhoeae | cdiA1 | Pangenome | 5'-AAGAAAGTCTTCAGCGAAAATGGTAAGTTG | 5'-CAAAGTATAATTTTGTTCACGATGCCCTGTTT | 5'-ATCATGTCCTTTACGACGCGCA |
g4966 | Pangenome | 5'-ATAGCTATGTTTCAACTTGTGAATTTGTAG | 5'-CAGATATAATCAAAATCTTCTTGTTGGTCA | 5'-GCCTTTGGGTTTACGTCCATAA | |
g2342 | Pangenome | 5'-GAATTGCAATTGGTTTCACACAAAGATGAG | 5'-TATCCAGCATCGCATAGCCTGATTCCAAGTTT | 5'-ACGTGGAACGAGTGCGTAGGCC | |
g2403 | Pangenome | 5'-CACTATCACATCGGCATTCCCAAATACAACG | 5'-TTATTTGGGAATATCGGGCAGGATAAAAGG | 5'-ATAAAGCCGTCGCAAAGGGTAT | |
g4212 | Pangenome | 5'-GTTAAATTTTTCAGGCGGTTCATTGGATTG | 5'-TATTTCTTTTCCCGAATCAATTTGTTGTAACG | 5'-CGGCTTTTCTTGAAGTTGATAA | |
g4380 | Pangenome | 5'-GAACAACTTTTAAACCACGCCGTCCGCTATG | 5'-TTTCGTGTGCCAGTTCTGCCAGCCCTCAATTT | 5'-GATTTCGAGCTTTCGGTAGTAT | |
porA | Pangenome | 5'-AATAATAATGTGGCTTCGCAATTGGGTATT | 5'-GCATATTTAAGGGCATAATTTCCGAAAAAG | 5'-ACTCGGAACAAATTGAATGCTG | |
Streptococcus pyogenes | g777 | Pangenome | 5'-CAAGTAAGTTTAATGATGATAGTGCTATTGTC | 5'-TACCACTACCAGTAGTATCAGTAAAATAAACA | 5'-ATTGACCTCGTGAAAACTTAGC |
pbp3 | Pangenome | 5'-CCATTTACAGTTATAATTACATGCTTAAAGGC | 5'-GTTGTTTTAAATATAGCTAAATCATCCTCGTG | 5'-ATTTTCGACACTAGTAGCTACA | |
g3729 | Pangenome | 5'-TACTATATACTAAAGGCTGTATGCGAATCAAT | 5'-TATCTGTTAAGGTAACCACATAGGCTCTTTTA | 5'-GTACAACAGTCATATTCCCCGA | |
g3664 | Pangenome | 5'-CTTAAATAGTGAACAAAAAGCTGTATCTGCTA | 5'-TAACCTTTTGGAGTCGAAATAATATCAATCTG | 5'-GGTAAGTTATTAGGAGTGAGTC | |
g3943 | Pangenome | 5'-GAAGAGATAGTAGGTTTTTCAACTCAAGAATA | 5'-GTAAGCTTCAACTAATTTAGGATAGACATCTT | 5'-AGACGGTTAAGCATCTTATACA | |
smc | Pangenome | 5'-GAAAAGAAATTTGAGCAAGCTAATCAATCTC | 5'-CATAGTCCTCTATTTCAAATCTTAATTGGACT | 5'-CCACTGGGGTAGTCTTTGAATG | |
ruvB | Pangenome | 5'-CGAATATATTGGACAAGATAAAGTTAAGGAAC | 5'-GGTCCAAATAATAAAACATGGTCTAAAGATTC | 5'-TAGAAGCAGCTAAAAGACGTGA | |
Neisseria gonorrhoeae | g686 | k-mer | N/A | N/A | 5'-AAATTTAAGGCCTAATTTGCCT |
g721 | k-mer | N/A | N/A | 5'-CCGAACAAGCGGTCCGGACTCC | |
g716 | k-mer | N/A | N/A | 5'-CCACTTATCCGCGCTTTCGCCA | |
g752 | k-mer | N/A | N/A | 5'-CACCAACGCCTGCTGTATAGCG | |
g588 | k-mer | N/A | N/A | 5'-ACCGGGCGGACAGCCGGTTCCG | |
g687 | k-mer | N/A | N/A | 5'-AACTTTTCCAGTGCGGCGAGAT |
