Fig. 1: Cyst fluid collection and electrolyte composition.
From: Electrolyte and metabolite composition of cystic fluid from a rat model of ARPKD
A Male and Female (10–12 weeks-old) PCK rat kidneys were flushed with PBS, sectioned, and cyst fluid was immediately carefully aspirated from intact cysts and snap-frozen. Blood from the abdominal aorta and urine directly from the bladder were also collected. B There is no significant difference in 2 kidney/total body weight ratios (2 K/TBW) at the collected age. C Cyst and Urine osmolalities (D) Na+ concentrations (E) K+ concentrations (F) Cl− concentrations (G) Ca2+ concentrations (H) Non-fasted glucose concentrations. Individual male (blue) and female (pink) data points are shown in each graph. Significance was determined by unpaired t-test (2 K/TBW, Osmolality) or Brown-Forsythe and Welch ANOVA with Dunnet’s correction p < 0.05 considered significant. N ≥ 5 rats. Graphs demonstrate the mean ± SEM. *p < 0.05 **p < 0.01 ***p < 0.001.
