Fig. 4: Primed cells have transient memory, and slow-growing cultures are not more prepared to survive Cip.
From: Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress
A Schematic of Memory Test, which measures the variation in persister percentages at the single cell level among clonal populations across two replicates. B P. megaterium primed cells exhibit a transient memory before antibiotic addition, with this memory being passed down for ~9 generations. Statistical analysis using a t-test indicated no apparent significant differences between the FT of the two replicates for each dilution (n = 48 for each replicate). Both the x- and y-axes are log10 scaled. C P. megaterium cultures with varying cell densities (as measured by CFU/mL and OD600), grown over the same duration, exhibited no correlation with persister percentages (indicated as % Survival). Additionally, no correlation was observed between the growth rates of individual clonal cultures or their lag periods with persister percentages (n = 36). The y-axis is a log10 scale.
