Fig. 1: Impact of host nitrate nutrition on Botrytis cinerea pectinolytic capacities and plant defense regulation.

A LC–MS quantitative analysis of OG production after incubation of spores from the WT strain of the fungus for 15 h with A. thaliana Col-0 leaves from plants cultivated at high or low N. The graph represents the peak area of each type of product over the total of all detected OGs expressed as %. B Transcript accumulation during infection in low N and high N conditions at 1-day post-inoculation on detached leaves are represented for plant defense genes. Plant gene targets were normalized with AtUBI4 and similar results were obtained using AtAPT1. Three independent experiments were conducted with similar results. Data are expressed as mean normalized expression in arbitrary units (a.u.) and are the means of triplicates (±SE). Statistical differences represented between high and low N are the results of an ANOVA followed by a Fisher’s LSD test in (A) and a two-sample t-test in (B): *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.