Fig. 1: Defined culture conditions promote greater uniformity among PSC lines compared to undefined culture conditions.

a Schematic representation of the three groups included in the analysis. b Table summarizing the exact culture conditions and number of PSC lines for each group. c PCA visualization of PSC lines derived and cultured in 1. Undefined culture conditions (in gray), 2. Defined culture conditions (in dark blue), 3. Transferred from undefined to defined culture conditions (in light blue) and 4. Fibroblasts (in green). d PCA visualization as in C, with ESCs (in orange) and iPSCs (in gray) highlighted. e PCA visualization as in D of ESC (in orange) and iPSC (in gray) lines derived and cultured in 1. UD culture conditions (in circles), 2. FD culture conditions (in triangles) and 3. Transferred from UD to FD culture conditions (in squares). Fibroblast samples were excluded from the visualization (f). PCA visualization of genetically identical PSC lines within dataset derived and cultured in 1. Undefined culture conditions (in squares), 2. Defined culture conditions (in circles) and 3. Transferred from undefined to defined culture conditions (in triangles). The color codes represent different technical or biological replicates. When there is a similar color code in one of the three groups (UD, FD, or T), the data originates from either different iPSC clones of the same individual or iPSCs from monozygotic twins. Of note, there is no overlap in biological replicate samples between the three culture condition groups.