Fig. 1: The dynamic activity of CeA^SST+ neurons in response to acute stress.

A Schematic diagram of AAV-EF1a-DIO-jGCamp7s injection into the CeA of SST-Cre mice. B Representative image showing the expression of AAV-EF1a-DIO-jGCamp7s in the CeA of SST-Cre mice. Scale bars: 200 μm. C Schematic of experimental setup of fiber photometry recording. Schematic of physical restraint (D), heatmap (E), and peri-event plot of the average Ca²⁺ transients (F) associated with physical restraint. The color scale indicates the ΔF/F (%). n = 35 trials from 4 mice. Schematic of rat bedding stress (G), heatmap (H), and peri-event plot of the average Ca²⁺ transients (I) associated with rat bedding stress. The color scale indicates the ΔF/F (%). n = 36 trials from 4 mice. Schematic of air puff stress (J), heatmap (K) and peri-event plot of the average Ca²⁺ transients (L) associated with air puff stress. The color scale indicates the △F/F (%). n = 34 trials from 4 mice. Schematic of cage change challenge (M), heatmap (N) and peri-event plot of the average Ca²⁺ transients (O) associated with cage change challenge. The color scale indicates the ΔF/F (%). n = 32 trials from 4 mice. Red vertical dashed lines indicate the time points of introducing different stressors.