Fig. 2: Effects of acute and 1-hour optogenetic activation of CeA SST+ neurons on sleep-wake behavior in mice.
From: Central amygdala somatostatin neurons modulate stress-induced sleep-onset insomnia
A Schematic diagram of AAV-EF1a-DIO-hChR2(H134R)-mCherry injection into the CeA of SST-Cre mice. B Representative image showing the expression of AAV-EF1a-DIO-hChR2(H134R)-mCherry in the CeA. Scale bars: 200 μm. C Schematic diagram of acute optogenetic stimulation in sleep-wake behavior experiment. D The typical examples of the NREM sleep to wakefulness transition during the yellow (top) or blue (bottom) light stimulation. E Sleep stages after yellow (left) or blue (right) light stimulation in AAV-EF1a-DIO-hChR2(H134R)-mCherry mice. F Possibility of NREM sleep, REM sleep and wakefulness before, during and after yellow (left) or blue (right) light stimulation. G Timeline of sleep-wake behavior recording experiment with 1-h optogenetic stimulation. Mice were stimulated with light (10 ms, 20 Hz, 30 s on, 30 s off) from ZT 2 to ZT 3. H Sleep latency after yellow or blue light stimulation. Paired t-test (n = 10). I Representative EEG power spectra, EEG/EMG traces and hypnogram after yellow light (top) or blue light (bottom) stimulation. J Time course of wakefulness, NREM sleep, and REM sleep following yellow and blue light stimulation of the CeASST+ neurons from ZT 2 to ZT 3. Two-way repeated-measures ANOVA, Bonferroni post-hoc comparison (n = 10). K Total time spent in each stage during the 1-h exposure to yellow or blue light stimulation. Paired t-test (n = 10).
