Fig. 4: Y chromosome loss in KDM5C mutant human ccRCC tumours.
From: KDM5C and KDM5D mutations have different consequences in clear cell renal cell carcinoma cells
a Expression levels of Y chromosome genes in normal male kidney and in KDM5C wild type or mutant male ccRCC, RNAseq2 quantification from the KIRC TCGA study. Statistical differences were assessed using a two-sided Student’s unpaired t-test with Welch’s correction. b mRNA expression levels (z-scores) of Y chromosome genes in human ccRCC cell lines. c KDM5C and KDM5D western blots in the indicated cell lines. d KDM5C and KDM5D western blots in the 786-O cells infected with lentiviruses expressing the indicated combinations of sgCtrl, sgKDM5C #4, sgKDM5C #5, sgKDM5D #2 and sgKDM5D #4 together with spCas9. Predicted molecular masses are shown. Full western blot scans including molecular mass standards are provided in Supplementary Fig. 6.
