Fig. 2: MiR-1 levels in the cancer field. | Communications Biology

Fig. 2: MiR-1 levels in the cancer field.

From: Cigarette smoke induces angiogenic activation in the cancer field through dysregulation of an endothelial microRNA

Fig. 2

A MiR-1/ reference gene levels were measured in the tumor (T), adjacent tissue (AT), and distant tissue (DT) samples from smokers and expressed as 2−∆Ct.(n = 12 patients, p-value = 0.0048, one-way ANOVA nonparametric Friedman test). B, C T and AT samples were divided into low miR-1( < median) and high miR-1( > median) and the corresponding survivals of the patients in each group were compared using Kaplan Meier analysis (B) T-low vs T-high patients (n = 72 subjects, p = 0.0403, Log-Rank Mantel Cox test) (C) AT-low vs AT-high patients (n = 58 subjects, p = 0.0357, Log-Rank Mantel Cox test). D–H The levels of miR-1 and PI3K pathway genes/reference gene were measured in AT samples (described in table S2) by qRT-PCR and expressed as Log 2−∆Ct. D Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha (PI3KCA), (Spearman r = −0.3491, P = 0.0235, n = 42), (E) Growth Arrest And DNA Damage Inducible Beta (GADD45B), (Spearman r = −0.513, P = 0.0023, n = 33), (F) Forkhead box protein O1 (FOXO1), (Spearman r = −0.3232, P = 0.0419, n = 40) (G) Mechanistic Target Of Rapamycin Kinase (mTOR1), (Spearman r = −0.3499, P = 0.0394, n = 35), and (H) AKT Serine/Threonine Kinase 2 (AKT2), (Spearman r = −0.6081, P < 0.0001, n = 40).

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