Fig. 6: The role of miR-1 in CS-induced angiogenesis. | Communications Biology

Fig. 6: The role of miR-1 in CS-induced angiogenesis.

From: Cigarette smoke induces angiogenic activation in the cancer field through dysregulation of an endothelial microRNA

Fig. 6

A EA.hy 926 were transfected with miR-1 or scrambled control RNA (ctrl) and exposed to 1% CSE for the indicated times. Cell numbers were determined and normalized to values at time ‘0’ (n = 6 from 2 experiments, *p = 0.039). B HUVECs were transfected with miR-1 (or ctrl) treated with CSE and phospho- and total- ERK and beta-actin visualized by Western blot. The top and bottom lines in the marker lane represent 50 and 37 KD bands. C, D Cells were transfected with antagomiRs (amiR-1 or control RNA, actrl), treated with CSE, and relative cell numbers measured as described in (A). C EA.hy 926 (n = 6, *p = 0.0022) (D) HUVECs (n = 9 from 2 experiments, *p = 0.011).

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