Fig. 2: Principal component analysis (PCA) of transcriptomes shows time-resolved organization of Vitis vinifera somatic embryogenesis.

V. vinifera developmental stages are shown in different colors, where shades of red represent early (EI–G2), shades of purple represent mid (H–T1), T2 and C1, and shades of green represent the late developmental stages (C2–JP). Replicates are in the same color and connected with lines. We performed transcriptome sequencing in n = 5 (C2 and EP stages) and n = 3 (the remaining 10 stages) biological replicates. Black arrows correspond to the experimental timeline of V. vinifera development that starts with EI and ends at JP. Developmental stages: early induction (EI), pre-globular stage (PG), globular stage 1 (G1), globular stage 2 (G2), heart stage (H), torpedo stage 1 (T1), torpedo stage 2 (T2), cotyledonary stage 1 (C1), cotyledonary stage 2 (C2), seedling (S), seedling with epicotyl (EP) and juvenile plant (JP).