Fig. 3: Reemergence of mGluR5 in ACC astrocytes after neuropathic pain responsible for promoting astrocytic Ca2+ signals. | Communications Biology

Fig. 3: Reemergence of mGluR5 in ACC astrocytes after neuropathic pain responsible for promoting astrocytic Ca2+ signals.

From: mGluR5-mediated astrocytes hyperactivity in the anterior cingulate cortex contributes to neuropathic pain in male mice

Fig. 3: Reemergence of mGluR5 in ACC astrocytes after neuropathic pain responsible for promoting astrocytic Ca2+ signals.

a Immunohistochemical images of astrocytes in the ACC of sham or CCI mice. Red indicates s100β, green indicates mGluR5, and blue indicates nuclei. b Quantification of mGluR5 fluorescence intensity in ACC astrocytes from sham and CCI mice (n = 10 slices from 5 sham mice, n = 10 slices from 5 CCI mice, sham (gray) = 11.7 ± 1.507 a.u., CCI (red) = 46.89 ± 5.56 a.u., p < 0.0001, un paired t-test). c Representative images displaying all AQuA-detected events from a 1-minute ex vivo astrocytic GCaMP7b Ca2+ imaging experiment. Upper: representative image before application of the mGluR5 antagonist MPEP; below: representative image after application of MPEP. Colors indicate detected events. Scale bar: 10 μm. d Graph depicting the frequency of astrocytic Ca2+ signals in the ACC of CCI mice before and after application of the mGluR5 antagonist MPEP (n = 6 cells from 3 mice; baseline (red) = 1.695 ± 0.2438 min/100 μm2, MPEP (blue) = 1.209 ± 0.1665 min/100 μm2, p = 0.0117, paired t-test). e Graph showing the amplitude of astrocytic Ca2+ signals in the ACC of CCI mice before and after application of the mGluR5 antagonist MPEP (n = 6 cells from 3 mice; baseline (red) = 1.119 ± 0.06624 ▵F/F, MPEP (blue) = 0.5810 ± 0.05255 ▵F/F, p = 0.0020, paired t-test). Error bars represent the mean ± SEM.

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