Table 1

Reagent type (species) or resource

Designation

Source or reference

Identifiers

Additional information

Strain

C57BL/6 male mice (mus musculus)

Hangzhou Ziyuan Laboratory

Animal Corporation (RRID:IMSR_JAX:000664)

N/A

Genetic reagent (virus)

AAV2/5 GfaABC1D jGCaMP7b

Brain Case

(http://www.braincase.cn/)

N/A

3 × 10¹² gc/ml

Genetic reagent (virus)

AAV2/5 GfaABC1D- iβARK-2A- mCherry

Brainvta

(https://www.brainvta.tech/)

N/A

5.94 × 10¹² gc/ml

Genetic reagent (virus)

AAV2/5 GfaABC1D- mCherry

Brainvta

(https://www.brainvta.tech/)

N/A

5.09 × 10¹² gc/ml

Genetic reagent (virus)

AAV2/5 GfaABC1D- shRNA-mCherry

Sunbio Medical Biotechnology

(http://www.sbo-bio.com.cn/)

5’-GCAGCTTGCATCGCCTATATC-3’

6.4 × 10¹² gc/ml

Genetic reagent (virus)

AAV2/5 GfaABC1D- scramble-shRNA-mCherry

Sunbio Medical Biotechnology

(http://www.sbo-bio.com.cn/)

5’-GTTCTCCGAACG

TGTCAC GTA-3′

5.1 × 10¹² gc/ml

Chemical compound, drug

picrotoxin

MedChemExpress (https://www.medchemexpress.com/)

N/A

100 μM

Chemical compound, drug

tetrodotoxin (TTX)

(R&D System)https://www.rndsystems.com/cn

N/A

1 μM

antibody

mouse monoclonal anti-s100β

Synaptic systems

Cat #287111

RRID:AB_2814886

1/1000

antibody

mouse

monoclonal anti-NeuN

Millipore Cat# MAB377

RRID: AB_2298772

1/500

antibody

Rabbit

monoclonal anti-mGluR5

CST Cat# 55920

RRID:AB_2734718

1/500

antibody

Rabbit

monoclonal anti-Iba1

FUJIFILM Wako Pure Chemical Corporation Cat# 019-19741

RRID:AB_839504

1/500

antibody

Rabbit

monoclonal anti-NG2

Millipore Cat# AB5320

RRID:AB_91789

1/250

software, algorithm

AQuA (Astrocyte Quantification and Analysis)

N/A

https://github.com/yu-lab-vt/AQuA?tab=readme-ov-file

software, algorithm

Coreldraw

Alludo

https://www.coreldraw.com

software, algorithm

Prism

Graphpad

https://www.graphpad.com/scientific-software/prism/

software, algorithm

Matlab

MathWorks

https://se.mathworks.com/products/matlab.htm

software, algorithm

Fiji/ImageJ

NIH

https://imagej.nih.gov/ij/