Fig. 6: ELF-1 directly regulates Rack1 expression in DSS colitis.

A Distribution of ELF-1 binding sites from ChIP-Seq analysis of colonic tissues of WT mice. B KEGG pathway enrichment analysis of ELF-1 binding genes (binding peaks located in gene promoter regions). C Venn diagram showing overlapping gene between ELF-1 binding genes with down regulated DEGs in colonic epithelial cells of DSS-treated Elf-1^−/− mice. D ChIP-seq peak for ELF-1 binding site in Rack1 gene. E Top 2 predicted ELF-1 binding motifs in Rack1 gene promoter with the most significant P values. F Rack1 expressions in colonic epithelial cells of DSS-treated WT and Elf-1^−/− mice. G Rack1 expressions in colonic epithelial tissues of normal and DSS-treated WT and Elf-1^−/− mice (n = 4). Data are shown as mean ± SD, representing one of at least three independent experiments. **P < 0.01.