Fig. 6: Effects of VA on SIRT3 and mitochondrial dynamics-related factors in CM (CT26)-treated C2C12 cells. | Communications Biology

Fig. 6: Effects of VA on SIRT3 and mitochondrial dynamics-related factors in CM (CT26)-treated C2C12 cells.

From: Regulating Sirtuin 3-mediated mitochondrial dynamics through vanillic acid improves muscle atrophy in cancer-induced cachexia

Fig. 6: Effects of VA on SIRT3 and mitochondrial dynamics-related factors in CM (CT26)-treated C2C12 cells.The alternative text for this image may have been generated using AI.

A Myofibers were immunostained with antibodies for MitoTracker and SIRT3, and counter-stained with DAPI for visualization of the cell nucleus (magnification ×400). Fluorescence intensity was quantified using ImageJ software and presented as bar graphs. B Intracelluar ATP levels were measured C Oxygen consumption was analyzed. D The mRNA expressions of Sirt3, Ppargc1a, Nfe2l2, Mfn1, Opa1, and Dnm1l were analyzed by Real-Time RT-PCR assays. Results were expressed relative to Gapdh. E Protein levels of SIRT3, PGC1α, NRF1, MFN1, and DRP1 were measured by western blot analysis. F Protein levels of OXPHOS complexes were measured by western blot analysis. Results were expressed relative to GAPDH. All values are the means ± S.E.M. of three or more independent experiments. Statistical differences were evaluated using an unpaired t-test and a subsequent post hoc one-tailed Mann–Whitney U test. #p < 0.05 vs. CM (-); *p < 0.05 vs. CM (CT26). VA vanillic acid, CM (CT26) CT26-derived conditioned medium.

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