Fig. 5: The migration of CD4+ T cells into the spleen white pulp was primarily dependent on the IFN-γ/IFN-γ receptor signaling pathway. | Communications Biology

Fig. 5: The migration of CD4+ T cells into the spleen white pulp was primarily dependent on the IFN-γ/IFN-γ receptor signaling pathway.

From: Invariant NK T cells counteract HCC metastasis by mediating the migration of splenic CD4+ T cells into the white pulp and infiltration of B cells

Fig. 5

A Investigation of IFN-γ, TNF-α, and IL-4 in iNKTs of indicated group through flow cytometry. B Statistical analysis of IFN-γ, TNF-α, and IL-4 MFI of iNKTs of indicated group. C Determination of expression of IFN-γ receptor and TNFR I in CD4+ T cells of indicated group through flow cytometry. D Statistical analysis of IFN-γ receptor and TNFR I MFI of CD4+ T cells of the indicated group. E We periplasmically transferred primary iNKT cells (1 × 105) derived from WT or IFN-γ−/− mice into the spleens of Ja18−/− mice, followed by establishment of the HCC-M mouse model. Three days later, the fluorescein-conjugated anti-mouse CD45 antibody was injected into the mouse tail veins 15 min before the mice were euthanized. CD4+ T cells in the red pulp were able to bind to the antibody, while CD4+ T cells in the white pulp were unable to bind to the antibody. Flow cytometry was performed to analyze the proportion of CD4+ T cells located in the white and red pulp. F Statistical analysis of (E). G Intravital microscopic observation of the spleen was performed 4 h after Hepa1-6 cell were injected orthotopically into the spleen of CXCR6Gfp/+, Jα18−/− and CXCR6Gfp/+ IFN-γ−/− mice. Fluorescein-conjugated anti-mouse CD4 and CD169 antibodies were then used to label CD4+ T cells (Red) and marginal zones (Blue). Scale bar, 100 µm. White pulps have been indicated with a white dashed line. H Statistical analysis of the number of CD4+ T cells in white pulp in indicated group. I Expression levels of Stat1 and J CD40L mRNA in CD4+ T cells of Jα18−/− mice and WT mice with HCC metastasis. K Flow cytometry analysis of expression levels of IL-4 in CD4+ T cells of HCC-m IFN-γ−/− mice. L Statistical analysis of the percentage of IL-4+ CD4+ T cells in the indicated group. The data shown was obtained three independent experiments with n = 5 mice per group. The data has been displayed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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