Fig. 5: RRS induces delayed upregulation of GHS-R1a expression in PrLαCaMKII+ neurons. | Communications Biology

Fig. 5: RRS induces delayed upregulation of GHS-R1a expression in PrLαCaMKII+ neurons.

From: Stress-induced GHS-R1a expression in medial prefrontal cortical neurons promotes vulnerability to anxiety in mice

Fig. 5

a The experimental design. b RT-qPCR assays of Ghsr1a expression in the PrL. One-way ANOVA with Dunnett’s multiple comparisons test, n = 4–5 mice per group. c RT-qPCR assays of Ghsr1a expression in the BLA. One-way ANOVA with Dunnett’s multiple comparisons test, n = 5 mice per group. d Representative FISH images showing Ghsr1a expression in PrLαCaMKII+ neurons. Scale bar, 10 μm in HM (high magnification), 20 μm in LM (low magnification). e Cell number and Ghsr1a expression quantification in FISH studies. Upper, Percentage of Ghsr1a+Camk2a+ neurons in total PrLCamk2a+ neurons. Lower, The average fluorescence intensity density (IntDensity) of Ghsr1a in indivadual Ghsr1a+Camk2a+ PrL neurons. Data were normalized by control group before comparison. One-way ANOVA with Dunnett’s multiple comparisons test, n = 4–5 mice per group (4–5 brain sections each mouse). All bar graphs represent mean ± SEM.

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