Fig. 7: Increasing GHS-R1a expression in PrLαCaMKII+ neurons enhances anxiety-like behavior. | Communications Biology

Fig. 7: Increasing GHS-R1a expression in PrLαCaMKII+ neurons enhances anxiety-like behavior.

From: Stress-induced GHS-R1a expression in medial prefrontal cortical neurons promotes vulnerability to anxiety in mice

Fig. 7

a Experimental design. This figure was created using BioRender(https://www.biorender.com/). b Viral infection images and RT-qPCR assays. Left, Representative images illustrating viral infection in the PrL. Scale bar, left: 500 μm, right: 200 μm. Right, RT-qPCR assays of Ghsr1a expression in the PrL. Unpaired t-test, n = 3 mice per group. c EPM and FS tests conducted at 6 d after RRS exposure. Unpaired t-test, n = 9–11 mice per group. d Experimental design. This figure was created using BioRender(https://www.biorender.com/). e–g Comparison of synaptic activity in virus-infected PrLαCaMKII+ neurons with and without RRS exposure. e mEPSCs. control: n = 12 cells, RRS: n = 10 cells. f sEPSCs. n = 13 cells per group. g sIPSCs. control: n = 16 cells, RRS: n = 10 cells. Unpaired t-test, control vs. RRS, 2 - 3 slices per mouse from 5 to 6 mice per group. All bar graphs represent mean ± SEM.

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