Fig. 4: Functional relationship among nematode SPI-I8, human RACK1 and MKRN1 in HEK 293 T cells. | Communications Biology

Fig. 4: Functional relationship among nematode SPI-I8, human RACK1 and MKRN1 in HEK 293 T cells.

From: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1

Fig. 4: Functional relationship among nematode SPI-I8, human RACK1 and MKRN1 in HEK 293 T cells.

a SPI-I8B of Haemonchus contortus (Hc-SPI-I8B) enhances the negative effect of HsRACK1 on the relative luciferase activity of NF-κB in HEK 293 T cells. b Human MKRN1 increases the relative luciferase activity of NF-κB and abolishes the inhibitory effect of HsRACK1 on NF-κB in HEK 293 T cells. c, d The promoting effect of MKRN1 on NF-κB is compromised by HsRACK1 and Hc-SPI-I8B. e Lentiviral RNA interference of HsMKRN1 coding gene, which f decreases the mRNA level of rack1, and g A20, IL-8, IκBα and TNF-α in HEK 293 T cells. h Schematic diagram showing the functional relationship among Hc-SPI-I8B, HsRACK1 and HsMKRN1 in HEK293T cells. Hc-SPI-I8 indirectly promotes the role of HsRACK1 in suppressing NF-κB signalling, possibly by hijacking or interfering HsMKRN1 from the HsMKRN1–HsRACK1 interaction. *p < 0.05; **p < 0.01; ****p < 0.001; ****p < 0.0001; ns, not significant.

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