Fig. 6: Nematode SPI-I8B decreases K63-dominant polyubiquitination at K183 and K185 of RACK1 in HEK 293 T cells. | Communications Biology

Fig. 6: Nematode SPI-I8B decreases K63-dominant polyubiquitination at K183 and K185 of RACK1 in HEK 293 T cells.

From: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1

Fig. 6: Nematode SPI-I8B decreases K63-dominant polyubiquitination at K183 and K185 of RACK1 in HEK 293 T cells.

a, b Myc-tagged human MKRN1 mediates K48 and K63 polyubiquitination at K183 and K185 residues of Flag-tagged RACK1 in HEK 293 T cells. c, d Overexpression of Haemonchus contortus SPI-I8B decreased the K48 and K63 polyubiquitination at K183 and K185 residues of Flag-tagged RACK1 in HEK 293 T cells. e, f Compared with wildtype ubiquitin, site mutation of K48R and K63R of ubiquitin decreased MKRN1-mediated K48 and K63 polyubiquitination of RACK1 in HEK 293T cells. g, h Overexpression of Hc-SPI-I8B decreased the K48(R) and K63(R) polyubiquitination at K183 and K185 residues of Flag-tagged RACK1 in HEK 293T cells. Tubulin is used as an internal control. Cells transduced with empty vector are used as negative control.

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