Fig. 1: PMCA4 is downregulated in HR+ breast cancer subtypes.
From: The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation
a PMCA4-specific antibody staining of normal and neoplastic LUMA, LUMB1 and LUMB2 subtype mammary gland tissue samples. GR1 or 2 indicates histological grade 1 or grade 2. b Fraction of cancer cells showing positive membranous PMCA4 staining; n(normal) = 4 (obtained after breast reduction surgery), n(LUMA) = 39, n(LUMB1) = 50, n(LUMB2) = 20 tissue samples. Error bars show 95% confidence intervals (CI). c Protein expression of PMCA4 (NP_001675.3:S328) and PMCA1 (NP_001673.2:S1182) in normal breast tissue and luminal breast cancer subtypes. Data derived from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) (http://ualcan.path.uab.edu) that are expressed as z-values and analyzed by Student’s t test; n(PMCA4b/normal) = 18, n(PMCA4b/luminal) = 64, n(PMCA1b/normal) = 18, n(PMCA4b/luminal) = 64 tissue samples; ***p < 0.001, nsp > 0.05; ns indicates non-significant difference. Error bars show standard deviations. d Kaplan–Meier relapse-free survival analysis in breast cancer patients with LUMA, LUMB1 or LUMB2 subtype tumors with low and high ATP2B4/PMCA4 and ATP2B1/PMCA1 expression levels. GR 2 indicates histological grade 2. Data were collected by the online survival analysis tool (http://www.kmplot.com) using microarray data analysis; n(ATP2B4/LUMA-GR2/low) = 97, n(ATP2B4/LUMA-GR2/high) = 297, n(ATP2B4/LUMB1/low) = 99, n(ATP2B4/LUMB1/high) = 301, n(ATP2B4/LUMB2/low) = 150, n(ATP2B4/LUMB2/high) = 108, n(ATP2B1/LUMA-GR2/low) = 257, n(ATP2B1/LUMA-GR2/high) = 254, n(ATP2B1/LUMB1/low) = 239, n(ATP2B1/LUMB1/high) = 161, n(ATP2B1/LUMB2/low) = 89, n(ATP2B1/LUMB2/high) = 169 patients.
