Fig. 4: PMCA4b mediates purinergic receptor-activated Ca2+ signaling in MCF-7 cells.
From: The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation
a Intracellular Ca2+ was measured with the R-GECO fluorescence indicator in parental, PMCA4 specific shRNA (sh-PMCA4), GFP-PMCA4b and GFP-PMCA4bLA-expressing MCF-7 cells. 10 μM ATP was added at the 120 s mark. Signals from individual cells are illustrated using different colored lines. Number of analyzed cells: n(MCF-7) = 12, n(sh-PMCA4) = 15, n(PMCA4b) = 18, n(PMCA4bLA) = 14. Data are derived from 2 independent experiments. b Area under curve (AUC) values were calculated from curves in panel (a) and analyzed with Kruskal–Wallis and Dunn’s multiple comparisons tests; adjusted p values: **p < 0.01, ***p < 0.001; non-significant difference is not labeled. Graph displays means with 95% confidence intervals. c First peak F/F0 maximum values were calculated from data in (a) and analyzed with Kruskal–Wallis and Dunn’s multiple comparisons tests; adjusted p values: **p < 0.01, ***p < 0.001; non-significant difference is not labeled. Error bars show 95% confidence intervals.
