Fig. 8: PMCA4b promotes central lumen formation in 3D cultures of MCF-7 cells.
From: The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation
a Phalloidin staining of mammospheres formed by parental, PMCA4-specific shRNA (sh-PMCA4), GFP-PMCA4b and GFP-PMCA4bLA-expressing MCF-7 cell lines cultured in Matrigel for 10 days. b Schematic figures demonstrating structures of mammospheres based on results on (a). c Statistical comparisons of the ratios of mammospheres with central lumen in 3D cultures between parental, PMCA4-specific shRNA (sh-PMCA4), GFP-PMCA4b- and GFP-PMCA4bLA-expressing MCF-7 cell lines cultured in Matrigel. Graph displays means with 95% confidence intervals, n = 8 images with 10–15 mammospheres per image for each cell type. Data were analyzed with ordinary one-way ANOVA and Tukey’s multiple comparisons tests, adjusted p values: nsp ≥ 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, non-significant differences are not labeled. d Transmission electron microscope images of 14 days old mammospheres formed by parental, PMCA4-specific shRNA (sh-PMCA4), GFP-PMCA4b and GFP-PMCA4bLA-expressing MCF-7 cell lines cultured in Matrigel. Insets show magnified areas indicated by the dashed black squares.
