Fig. 1: SUN1/2 depletion alters nuclear morphology.
From: Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis
a Representative images of MSCs treated with siRNA targeting SUN1/2 (siSUN) which were stained for SUN1 (green), SUN2 (red), and DNA (blue). b siSUN-treated cells had 47% decrease of SUN1 intensity (n = 1478, P < 0.0001). c SUN2 intensity levels were decreased by 52% in siSUN-treated cells (n = 1478, P < 0.0001). d MSCs treated with siSUN had an increase in nuclear area by 7% (n = 1478, p 0 < 0.01). e Nucleus perimeter decreased in siSUN-treated MSCs by 8% (n = 1478, p < 0.0001). f Nuclear circularity decreased by 9% in siSUN-treated cells (n = 1478, p < 0.001). Comparisons were made against control using non-parametric Mann–Whitney test where *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The scale bar represents 50 μm. Results were presented as mean ± STD.
