Fig. 5: dnKASH expression induces accelerated adipogenesis in MSCs.
From: Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis
a Representative western images of doxycycline-induced dnKASH cells and control cells grown in growth media and adipogenic media. b During adipogenesis, doxycycline-treated samples had 98% increased levels of Adipoq (n = 3, P < 0.01). c Cepba experienced an increase of 27% but was not significant during adipogenesis in doxycycline treatment (n = 3, p = 0.38). d Pparg in the doxycycline treatment group during adipogenesis increased by 90% (n = 3, P < 0.05). e Representative photos of lipid droplets (green) and DNA (blue). f Quantification of mean lipid droplet intensity per cell in each field of view showed an increase of lipid droplet amounts in doxycycline-treated cells by 258% (n = 50, P < 0.05) during adipogenesis. Western analysis group comparisons were one-Way Anova. Lipid droplet Intensity group comparisons were made using the Kruskal–Wallis test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The scale bar represents 50 μm. The scale bar represents 50 μm. Results were presented as mean ± STD.
