Fig. 7: Global levels of H3K9me3 and enrichment on Adipoq increase during SUN1/2 depletion.

a Representative western images of heterochromatin markers H3K9me3 and H3K27me3 and euchromatin marker H3K4me3 in siSUN and siCntl treatments during growth in growth media and adipogenic media. b Western analysis of heterochromatin marker H3K9me3 revealed an increase of 56% in siSUN cells compared to siCntl during adipogenesis (n = 3, P < 0.05). c H3K27me3 had a decrease of 48% in siSUN cells compared to siCntl cells during adipogenesis (n = 3, P < 0.05). d Euchromatin marker H3K4me3 experienced no significant changes in global protein levels between siSUN and siCntl-treated cells during adipogenesis. e Representative images of siSUN and siCntl-treated cells grown in adipogenic media and stained for H3K9me3 (green) and Hoechst 33342 (blue). f H3K9me3 foci count per cell in siSUN cells compared to siCntl cells in growth media increased by 9% (n = 338, P < 0.01). g H3K9me3 foci area increased by 7% in siSUN cells compared to siCntl in growth media (n = 14,560, P < 0.001). h Representative images of siSun and siCntl treated cells grown in adipogenic media and stained for H3K9me3 (green) and Hoechst (blue). i H3K9me3 foci count per cell in siSUN cell compared to Sicntl Cells during adipogenesis increased by 43% (n = 213, P < 0.0001). j No detectable increase of H3K9me3 foci area was found in siSUN cells during adipogenesis (n = 8460). k CUR&RUN-qPCR targeting H3K9me3 localization on Adipoq showed an increase of 156% in siSUN cells compared to siCntl (n = 3, P < 0.05). Western analysis group comparisons were made using one-way ANOVA. H3K9me3 Foci count, and area comparisons were made using the Mann–Whitney test. CUR&RUN-qPCR comparisons were done using a one-tailed students t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The scale bar represents 25 μm. The scale bar represents 50 μm. Results were presented as mean ± STD.