Fig. 2: Mouse embryo implantation and trophoblast cell migration were promoted after melatonin treatment in vitro. | Communications Biology

Fig. 2: Mouse embryo implantation and trophoblast cell migration were promoted after melatonin treatment in vitro.

From: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

Fig. 2: Mouse embryo implantation and trophoblast cell migration were promoted after melatonin treatment in vitro.

Mouse E4.5 embryos were randomly assigned to the melatonin group (10 μM, n = 20) and the control group (n = 20) and co-cultured with the Ishikawa cells (A). Embryonic attachment (B) and trophoblast cell migration (C) were assessed at E5.5. The migration range of embryonic trophoblast cells in the control group (D) and the melatonin treated group (E) were evaluated at E6.5. The white dotted line is the independent boundary. Statistical analysis of E5.5 embryo implantation rate (F), E5.5 embryo migration rate (G), and E6.5 trophoblast cell migration range (H). Error bars indicate standard deviation (SD). Scale bar is 50 μm, * indicates p < 0.05.

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