Fig. 5: Melatonin can attenuate H2O2-induced oxidative stress damage in HTR-8/SVneo cells. | Communications Biology

Fig. 5: Melatonin can attenuate H2O2-induced oxidative stress damage in HTR-8/SVneo cells.

From: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

Fig. 5: Melatonin can attenuate H2O2-induced oxidative stress damage in HTR-8/SVneo cells.

A microplate reader was used to detect ROS levels in HTR-8/SVneo cells after treatment with different concentrations of melatonin for 24 h (A) or H2O2 for 2 h (B). Cell viability was detected after treatment with each concentration of H2O2 for 2 h (C). The cellular ROS levels was detected in cells after the treatment of different concentrations of melatonin for 24 h and 800 μM H2O2 for another 2 h (D). Flow cytometry was used to detect the ROS levels of cells in each treatment group, and the ROS fluorescence intensity was statistically analyzed (E–I). Ns indicates no statistical difference, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

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