Fig. 8: The mouse in vivo embryo implantation model was used to explore the involvement of GDF15 pathway in the regulation of embryo implantation by melatonin. | Communications Biology

Fig. 8: The mouse in vivo embryo implantation model was used to explore the involvement of GDF15 pathway in the regulation of embryo implantation by melatonin.

From: Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

Fig. 8: The mouse in vivo embryo implantation model was used to explore the involvement of GDF15 pathway in the regulation of embryo implantation by melatonin.

Melatonin (10 mg/kg) and luzindole (1 mg/kg) were injected intraperitoneally into the mice on the day the plug was seen for 7 days. The uterus was harvested at E7.5 and the embryos were collected. Observe the embryo implantation sites of mice in each group (A), and perform statistical analysis on the number of uterine implantation sites (B). Immunohistochemical stain was used to analyze the localization and expression of GDF15, N-CAD and E-CAD in E7.5 embryonic and decidual tissues of mice in each group (C). E7.5 mouse embryos from each group were obtained and the protein expression levels of GPX4, GDF15, p-SMAD3, SMAD2/3, N-CAD and E-CAD were detected (D) B-ACTIN was the internal reference protein. Ns indicates no statistical significance, * indicates p < 0.05, ** indicates p < 0.01.

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