Fig. 6: Binding of GBP5 to STAT1 promotes the nuclear translocation of STAT1.
From: STAT1 mediates the pro-inflammatory role of GBP5 in colitis
a Immunofluorescence staining of THP-1 cells untreated or treated with IFNγ/LPS using antibodies against GBP5 and STAT1. Scale bar: 5 μm. b Subcellular fractionation analysis of GBP5 and STAT1. WT and GBP5-/- THP-1 cells were untreated or treated with IFNγ/LPS for 16 h, harvested to prepare nuclear and cytoplasmic fractions, and analyzed by Western blot with antibodies against GBP5, STAT1, and p-STAT1 (S727). GAPDH and Lamin B were used as internal references. The bar plot on the right was the quantification of three independent experiments. n = 3 biologically independent experiments for each group. Data are mean ± SEM, paired student t-test. c GBP5-STAT1 proximity ligation assay with THP-1 cells untreated or treated with IFNγ/LPS. Each red spot represents an interaction. Scale bar: 2 μm. Nuclei were stained with DAPI (blue).
