Table 2 Parameters associated with cleaved, solubilized HIV-1 Env trimers
HIV-1 Env | AD8 | AE2 | Tri FPPR.2 | Tri FPPR.1 | ||||
|---|---|---|---|---|---|---|---|---|
Parameter | ||||||||
Solubilization and preparation | SMA Non-crosslinked BMS-806 | SMA Crosslinked BMS-806 | A18 Non-crosslinked BMS-806 | A18 Non-crosslinked BMS-806 | ||||
Interprotomer opening angle (degrees)* | gp41 | gp120 | gp41 | gp120 | gp41 | gp120 | gp41 | gp120 |
Protomers 1 and 2 | 123.6 | 123.0 | 124.3 | 122.8 | 125.6 | 124.5 | 123.2 | 123.0 |
Protomers 2 and 3 | 129.2 | 124.9 | 128.2 | 124.4 | 125.1 | 122.6 | 128.7 | 125.1 |
Protomers 1 and 3 | 107.3 | 112.1 | 107.8 | 112.7 | 108.0 | 113.0 | 107.8 | 112.0 |
Shift in protomer angles from a C3-symmetric trimer (degrees) | gp41 | gp120 | gp41 | gp120 | gp41 | gp120 | gp41 | gp120 |
Protomer 2 | 3.6 | 3.0 | 4.2 | 2.9 | 6.3 | 4.5 | 3.4 | 3.0 |
Protomer 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Protomer 3 | 12.7 | 7.9 | 12.2 | 7.3 | 12.0 | 7.0 | 12.2 | 8.0 |
gp41 HR1N conformation | ||||||||
Protomer 2 (chain D) | Helix | Helix | Helix | Strong helix | ||||
Protomer 1 (chain B) | Helix | Helix | Helix | Weak helix | ||||
Protomer 3 (chain F) | Unmodelled disorder | Loop | Loop | Loop | ||||
Tilt of α9 helix: Centroid distance (Å) from chain F | ||||||||
chain F | 0 | 0 | 0 | 0 | ||||
chain D | 1.5 | 1.2 | 0.9 | 1.4 | ||||
chain B | 2.2 | 2.0 | 1.3 | 1.1 | ||||
MPER density (last resolved gp41 residue) | ||||||||
Protomer 3 (chain F) | No connectivity (657) | No connectivity (661) | Weak density (662) | Weak density (662) | ||||
Protomer 2 (chain D) | No connectivity (657) | No connectivity (661) | Weak density (662) | Complete trace (685) | ||||
Protomer 1 (chain B) | Strong connectivity but no MPER order (657) | Strong connectivity but no MPER order (664) | Complete trace (689) | Partial trace (669) | ||||
