Fig. 1: FXRE and ligand co-regulate SRC1 binding to FXR-RXRα. | Communications Biology

Fig. 1: FXRE and ligand co-regulate SRC1 binding to FXR-RXRα.

From: Structural basis for the asymmetric binding of coactivator SRC1 to FXR-RXRα and allosteric communication within the complex

Fig. 1: FXRE and ligand co-regulate SRC1 binding to FXR-RXRα.

A Schematic diagram of the domain architecture of SRC1, FXR and RXRα. Truncations utilized in the experiments are indicated. B The FXR response element in the promoter of the FXR target gene hSHP-1 used in the experiment, comprising AGGTCA inverted repeats separated by a nucleotide. C Binding profiles of different states of FXR120-RXRα98 to SRC1630-987 in a concentration gradient. Biotin-labeled SRC1630-987 was immobilized on the sensor, and the effects of FXR and RXRα agonists, as well as FXRE (hSHP-1), on its binding affinity to FXR120-RXRα98, were examined. The KD values are also presented in the table.

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