Fig. 3: Markers of mature ciliated cells are enriched approaching the fimbria. | Communications Biology

Fig. 3: Markers of mature ciliated cells are enriched approaching the fimbria.

From: Spatial transcriptomic profiling of the human fallopian tube epithelium reveals region-specific gene expression patterns

Fig. 3

a Diagram showing differential expression of markers of mature ciliated cells along the fallopian tube. Volcano plots comparing expression of transcripts between fimbria and isthmus sections in the discovery (b) and validation (c) cohorts, with mature ciliated cell markers31 highlighted in green. Red lines: p-value cutoff (\({-{{\mathrm{log}}}}_{10}(p)=1.3\)); blue lines = FC cutoff (\({{{\mathrm{log}}}}_{2}({FC})\,=\,+/-\,0.5\)). All p-values are calculated using t-test.) d Boxplot showing ratio of FOXJ1:PAX8 ratio in ciliated cells (discovery cohort, n = 35). e Boxplot showing PAX8:FOXJ1 ratio in secretory cells (discovery cohort, n = 42). Boxplots showing expression of mature ciliated cell markers MLF1 (f), SPA17 (g), CTSS (h), and C6 (i) in ciliated and secretory segments (discovery cohort). j-o Boxplots plotting the same ratios or gene expression changes as (d-i), but for the validation cohort (n = 36 ciliated and n = 38 secretory segments). Respectively, PAX8:FOXJ1 ratio in ciliated cells (j), PAX8:FOXJ1 ratio in secretory cells (k), MLF1 expression (l), SPA17 expression (m), CTSS expression (n), and C6 expression (o). Lower and upper hinges of boxplot correspond to first (Q1) and third quartiles (Q3); central line corresponds to median. Whiskers of all boxplots range from Q3 + 1.5×IQR to Q1-1.5×IQR. All p-values calculated using two-sided t-test. Created in BioRender. Sipes, J. (2025) https://BioRender.com/p37o480.

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