Fig. 7: HIF1A upregulates its target genes by enhancing H3K27ac modifications at their promoters or enhancers. | Communications Biology

Fig. 7: HIF1A upregulates its target genes by enhancing H3K27ac modifications at their promoters or enhancers.

From: HIF1A facilitates hypoxia-induced changes in H3K27ac modification to promote myometrial contractility

Fig. 7

a RT-qPCR verification of the expression of some HIF1A target genes. n = 3. Data are represented as mean ± SD. *, ***, and **** indicate p < 0.05, p < 0.001, and p < 0.0001, respectively. b ChIP-qPCR verification of HIF1A binding at the promoters (suffix with “p”) or enhancers (suffix with “e”) of partial HIF1A target genes. n = 4. Data are represented as mean ± SD. A non-HIF1A peak region was set as a negative control (Neg). * and **** indicate p > 0.05 and p-value < 0.0001, respectively. c The interaction between HIF1A and P300 proteins was confirmed through Co-immunoprecipitation (Co-IP) followed by Western blot analysis. d The interaction between HIF1A and P300 proteins was validated using a GST pull-down assay followed by Western blot analysis. e ChIP-qPCR detection of H3K27ac modifications changes at the promoters or enhancers of partial HIF1A target genes after HIF1A knockdown. n = 4. Data are represented as mean ± SD. A non-HIF1A peak region was set as a negative control (Neg). ns, **, and **** indicate p > 0.05, p < 0.01, and p < 0.0001, separately. f Cell adhesive properties in si-HIF1A and negtive control (NC) hMSMCs. The y-axis represents the cell index. n = 3. Data are represented as mean ± SD. **p < 0.01.

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