Fig. 4: Chemical-inducible cGAS phase separation and signaling in cells.
From: Rational design of chemical- and light-inducible cGAS activation based on mechanistic insights
A–C Rapamycin induced phase separation of EGFP-FKBP-proteins and mCherry-cGAS-FRB in HEK293T cells. Scale bar: 10 μm. D–F Plots of normalized fluorescence intensity of FKBP-proteins and cGAS-FRB over 30 min time course after adding rapamycin. Data are collected from 3 cells. G-I Counts of intracellular puncta with or without rapamycin. Box plots depict the first quartile and third quartile with median as center and min-max values for whiskers, hollow squares represent average values, except for outliers calculated as data points ±1.5× interquartile range. (N = 10) J–L Bar graphs showed the relative abundance of phosphorylated IRF3 and TBK1 to total IRF3 and TBK1 proteins analyzed by Western blotting shown in Fig. S5A–C. (N = 3) The time points of (A–C) were initiated from the inclusion of rapamycin. Data are presented as mean ± SEM. Statistical significance was assessed by two-tailed unpaired t test in (G-L). Statistical differences were represented as follows: ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001.
