Fig. 3: The complex of αSyn amyloid fibrils and ΔJ-DNAJB1. | Communications Biology

Fig. 3: The complex of αSyn amyloid fibrils and ΔJ-DNAJB1.

From: Stepwise recruitment of chaperone Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibril disassembly

Fig. 3

A Micrograph showing the fuzzy decoration of ΔJ-DNAJB1 on αSyn amyloid fibrils. ΔJ-DNAJB1 repeats are discernible as dark dots. Scale bar, 50 nm. B 2D class and FT showing the 40 Å repeat in ΔJ-DNAJB1 decoration, shown in reversed contrast. Scale bar, 50 Å. C Side view of the aligned 2D classes and calculated cross-section. Scale bar, 100 Å. D 3D reconstruction of the ΔJ-DNAJB1: αSyn fibril complex. The density corresponding to ΔJ-DNAJB1 was fitted with the crystal structure of the truncated human DNAJB1 lacking the J-domain and G/F linker (PDB: 3agy) using Flex-EM. Scale bar, 50 Å.

Back to article page