Table 6 Details of tilt series data collection
Datasets | WT DNAJB1 dataset 1 | WT DNAJB1 dataset 2 | ΔH5 DNAJB1 |
|---|---|---|---|
Microscope | FEI Titan Krios III | FEI Titan Krios III | FEI Titan Krios III |
Acceleration voltage (kV) | 300 | 300 | 300 |
Camera | Selectris Falcon IV | Selectris Falcon IVi | Selectris Falcon IVi |
Energy filter slit width (eV) | 5 | 3 | 3 |
Pixel size (Å) | 1.9 | 1.94 (rounded to 1.9) | 1.94 (rounded to 1.9) |
Total dose (e/Å2) | 118 | 116 | 116 |
Defocus range (μm) | -2 to -6.5 | -2 to -4.4 | -2 to -4.4 |
Number of movies collected | 3261 | 2797 | 13429 |
