Fig. 1: Tlp7.6 interacts with CYP38 and localizes to the thylakoid lumen. | Communications Biology

Fig. 1: Tlp7.6 interacts with CYP38 and localizes to the thylakoid lumen.

From: Characterization of Arabidopsis thaliana thylakoid lumen 7.6 protein functions in photosystem II assembly

Fig. 1: Tlp7.6 interacts with CYP38 and localizes to the thylakoid lumen.

a Co-immunoprecipitation (Co-IP) assay shows the interaction between TLP7.6 and CYP38. Thylakoid membranes from Col-0, and transgenic cyp38-2 mutants expressing 35S-driven C-terminal His-tagged CYP38 were solubilized with 0.1% (w/v) n-dodecyl-β-D-maltoside (β-DM), incubated with Ni-NTA Agarose, and eluted proteins analyzed by immunoblotting with TLP7.6 and His antibodies. b GFP signals show that the TLP7.6-GFP fusion protein expressed from the 35S::TLP7.6-GFP construct localized to the chloroplast. Green fluorescence indicates GFP, red fluorescence shows chloroplast autofluorescence, and yellow fluorescence represents the merged signals. Scale bar: 25 µm. c Immunoblot analysis of chloroplast components. Thylakoid membrane, thylakoid lumen and stroma fractions from Col-0 were probed with antibodies against D1, ClpC, PC, and TLP7.6. d Tissue-specific expression of TLP7.6. qRT-PCR analysis of TLP7.6 transcript levels in roots, stems, young leaves, and old leaves of Col-0. Error bars: mean ± SE (n = 3).

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