Fig. 7: BN-PAGE and analysis of thylakoid membrane complexes via 2D SDS-PAGE. | Communications Biology

Fig. 7: BN-PAGE and analysis of thylakoid membrane complexes via 2D SDS-PAGE.

From: Characterization of Arabidopsis thaliana thylakoid lumen 7.6 protein functions in photosystem II assembly

Fig. 7: BN-PAGE and analysis of thylakoid membrane complexes via 2D SDS-PAGE.The alternative text for this image may have been generated using AI.

a Thylakoid membranes from plants grown under normal light conditions (60 μmolm-²s-¹) for 3 weeks. b Thylakoid membranes from plants grown under low light conditions (15 μmolm-²s-¹) for 3 weeks. a-b Thylakoid membranes (1 μg of chlorophyll) were solubilized with 1% w/v β-DM and separated by 5–13.5% acrylamide gradient gel. PSI-M, PSI monomer; PSII-D, PSII dimer; LHCII, PSII light-harvesting complex; LHCII-T, PSII light-harvesting complex trimer. c–e Native gel strips from thylakoid membrane complexes of Col-0, cyp38-2 and cyp38-2/tlp7.6-1 were subjected to 12% SDS-PAGE and stained with silver. f Thylakoid membranes solubilized by 1% w/v β-DM were separated by BN-PAGE (1 μg chlorophyll each lane). The individual lanes were placed on 12% SDS-PAGE, followed by immunoblotting with antibodies against D1, LHCII and CP47.

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