Fig. 6: Structural modeling and validation of the NSP13-USP13 role in SARS-CoV-2 replication.
From: Multimodal SARS-CoV-2 interactome sketches the virus-host spatial organization
a Modeling of the USP13-NSP13 complex by Alphafold-Multimer and homology-based prediction (b). c Inhibition curve of SARS-CoV-2 infection in the presence of Spautin-1, using the nanoluciferase complementation assay. Briefly, a co-culture of Vero E6-NanoLg and Vero E6-NanoSm, plated at equal density the day before infection, was infected at a MOI of 0.01 with the Wuhan strain of SARS-CoV-2. Increasing concentrations of Spautin-1 (red) and GC376 (blue) were added at the time of infection. Luciferase was measured 24 h post-infection as a read-out of infection-induced syncytia formation (n = 4 biologically independent samples).
