Fig. 7: Comparison of growth, glycerol consumption and GK activities in S. acidocaldarius parental (MW00G), GK deletion and complementation strains.

a Growth comparison of the parental strain MW00G with the single deletion strains ∆saci_1117 and ∆saci_2033, and the double deletion strain ∆saci_1117∆saci_2033. Cells were cultivated in Brock’s basal medium containing 10 mM glycerol as the sole carbon and energy source. b Glycerol consumption of the different strains. c Growth and (d) glycerol consumption of the single deletion strains ∆saci_1117 and ∆saci_2033, complemented in trans by ectopic integration of the wildtype genes saci_1117 and the saci_2033-34 operon, respectively, into the upsE gene locus (saci_1494) under control of their native promoters. For comparison, the growth of the single deletion strains and the parental strain as in (a) is included. GK activity of the parental MW00G, deletion mutants, and complementation strains was measured and is presented in Supplementary Fig. 8. The lower panel presents the colour code and symbols used throughout the figure. Experiments were conducted in triplicate (n = 3, biological replicates) and error bars indicate the SD of the mean and individual data points are shown as grey dots.