Fig. 4: Increased pyroptosis in MAIT cells was shown in HFRS patients. | Communications Biology

Fig. 4: Increased pyroptosis in MAIT cells was shown in HFRS patients.

From: Hantaan virus infection induces human mucosal-associated invariant T cell pyroptosis through IRE1α pathway

Fig. 4: Increased pyroptosis in MAIT cells was shown in HFRS patients.

A Representative confocol immunoflurescent images and (B) quantification of pyroptotic MAIT cells showed the expression of cleaved N terminal Gasdermin D (green) in MAIT cells of HFRS patients and normal controls (N = 5). HLA-I staining indicated the cell membrane (red). DAPI staining identifies nucleus (blue). Merge images showed the co-expression of cleaved N terminal-gasdermin D with the cell membrane. Scale bar=20 μm, zoom scale bar=5 μm. C Representative image of ImageStream flow cytometric analysis showed MAIT cells from different samples. MAIT cells were stained with FLICA-caspase-1 (green), Vα7.2 (yellow), CD3 (blue), CD161 (purple), and CD8 (red) (N = 5). D Statistical analysis showed the percentage of cells with misshapen membrane. *p < 0.05, **p < 0.01.

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