Fig. 3: HKDC1 promotes immune escape of OC cells in vitro. | Communications Biology

Fig. 3: HKDC1 promotes immune escape of OC cells in vitro.

From: HKDC1 promotes ovarian cancer progression through boosting lipid metabolism and immune escape by stabilizing G6PC/G6PC2

Fig. 3

A Flow cytometry was employed to investigate the proportions of T cells (CD45+CD4+/CD8+), Treg cells (CD4+CD25+FOXP3+), and NK cells (CD3−CD16+CD56+) within PBMCs co-cultured with HKDC1-knockdown SKOV3 cells or HKDC1-overexpressing HEY cells. B Flow cytometry analysis was conducted to assess the expression levels of PD-1 on T cells and NK cells and measured IFN-γ production within CD4+/CD8+ T cells from PBMCs co-cultured with HKDC1-modulated SKOV3 and HEY cells. C qRT-PCR was used to quantitatively analyze PD-1 mRNA expression in T cells and NK cells from PBMCs co-cultured with HKDC1-modulated SKOV3 and HEY cells. D ELISA was performed to measure the levels of GZMB and perforin within NK cells from PBMCs co-cultured with HKDC1-modulated SKOV3 and HEY cells. *P < 0.05, ***P < 0.001, ****P < 0.0001, one-way ANOVA test. n = 3 biologically independent samples. Data are expressed as mean ± standard deviation.

Back to article page