Fig. 7: HKDC1 promotes immune escape by interacting with G6PC/G6PC2.

A Flow cytometry was employed to evaluate the proportions of T cells (CD45⁺CD4⁺/CD8⁺), Treg cells (CD4⁺CD25⁺FOXP3⁺), and NK cells (CD3⁻CD16⁺CD56⁺) within PBMCs co-cultured with HKDC1-knockdown SKOV3/ID8 cells and G6PC/G6PC2-overexpressing cells, as well as HEY cells with HKDC1 overexpression and G6PC/G6PC2 knockdown. B PD-1 expression in T cells and NK cells and IFN-γ levels in CD4⁺/CD8⁺ T cells were assessed by flow cytometry in PBMCs co-cultured under the same conditions. C qRT-PCR was used to analyze PD-1 mRNA expression in T cells and NK cells co-cultured with HKDC1-knockdown and G6PC/G6PC2-overexpressing SKOV3/ID8 cells, as well as with HKDC1-overexpressing and G6PC/G6PC2-knockdown HEY cells. D ELISA analysis was performed to measure the levels of GZMB and perforin in NK cells within PBMCs co-cultured with SKOV3 and HEY cells (exhibiting respective modulation of HKDC1 and G6PC/G6PC2) under the same conditions. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, one-way ANOVA test. n = 3 biologically independent samples. Data are expressed as mean ± standard deviation.