Fig. 4: Irradiation with 300 mJ/cm2 UVB but not UVA activated p38 MAPK and pro-inflammatory response in HCT-treated skin biopsies.
From: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies
A Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in untreated control biopsies (Ctrl) and in biopsies treated with HCT 6 h after irradiation with 300 mJ/cm2 UVA or UVB. Unirradiated biopsies served as group-specific control (non). B Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. C Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha (TNFα) and Interleukin 6 (IL6) and (D) Connective Tissue Growth Factor (CTGF) normalized against Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) 6 h after irradiation. E Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in Ctrl, or HCT 24 hours after irradiation with 300 mJ/cm2 UVA or UVB. Unirradiated biopsies served as group-specific control. F Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. G Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha (TNFα) and Interleukin 6 (IL6) and (H) Connective Tissue Growth Factor (CTGF) normalized against GAPDH 24 h after irradiation. For (B, C, D, F, G, H) n = 6 biopsies per group. Data are shown as mean ± SEM, with individual data points. For comparison of three groups P-value was determined using Kruskal-Wallis with Dunn´s multiple comparisons test for Ctrl group in (B and F) and an One-way ANOVA with Tukey multiple comparisons test for HCT group in (B and F). For (C, D, G and H) an One-way ANOVA with Tukey multiple comparisons test was used. IOD: Integrated optical density. Numerical source data are provided within the Supplementary Data 1 file.
